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以吉拉德试剂T作为NMR探针研究人源细胞色素c氧化修饰的影响因素

张广庆1,2，占建华1,2，肖雄1,2，朱勤俊1，蒋滨1,2,3,4，刘买利1,2,3,4，张许1,2,3,4*   

  1. 1. 磁共振波谱与成像全国重点实验室，中国科学院精密测量科学与技术创新研究院，湖北 武汉 430071；2. 中国科学院大学，北京100049；3. 华中科技大学武汉光电国家实验室，湖北 武汉 430074；4. 湖北光谷实验室，湖北 武汉 430074
  • 收稿日期:2025-04-27 修回日期:2025-05-12 出版日期:2025-05-12 在线发表日期:2025-05-12
  • 通讯作者: 张许 E-mail:zhangxu@wipm.ac.cn

Investigating the factors influencing oxidative modification of human cytochrome c using Girard's reagent T as an NMR probe

ZHANG Guangqing1,2，ZHAN Jianhua1,2，XIAO Xiong1,2，ZHU Qinjun1，JIANG Bin1,2,3,4，LIU Maili1,2,3,4，ZHANG Xu1,2,3,4*   

  1. 1. State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, Innovation Academy for Precision Measurement of Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China; 2. University of Chinese Academy of Sciences, Beijing 100049, China; 3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China; 4. Optics Valley Laboratory, Wuhan 430074, China
  • Received:2025-04-27 Revised:2025-05-12 Published:2025-05-12 Online:2025-05-12
  • Contact: ZHANG Xu E-mail:zhangxu@wipm.ac.cn

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摘要：

细胞色素c（Cytochrome c, Cyt c）的氧化修饰可能对蛋白自身的局部构象造成影响，但Cyt c结构的改变如何影响其氧化修饰程度尚不清楚. 本文以吉拉德试剂T（Girard’s Reagent T，GRT）作为核磁共振（NMR）探针，研究了人源Cyt c不同条件下的氧化修饰程度. 实验结果表明，通过还原甲基化的方法保护蛋白中的赖氨酸，可以降低蛋白的氧化修饰程度；Cyt c发生部分去折叠后，其氧化修饰程度会升高；Cyt c与心磷脂结合后可以很大程度提高蛋白的氧化修饰程度，但蛋白质聚集等其它因素可能对其氧化修饰有抑制作用.

关键词: 细胞色素c, 氧化修饰, 吉拉德试剂T, 甲基化, 核磁共振

Abstract:

 Oxidative modification of cytochrome c (Cyt c) may influence the local conformation of the protein, yet the mechanism by which structural alterations of Cyt c affect its degree of oxidative modification remains unclear. In this study, Girard’s reagent T (GRT) was employed as a nuclear magnetic resonance (NMR) probe to investigate the oxidative modification levels of human Cyt c under varying environmental conditions. Experimental results demonstrated that protecting lysine residues through reductive methylation effectively reduced protein oxidation. Partial unfolding of Cyt c was found to enhance its oxidative modification, while binding with cardiolipin significantly increased the extent of oxidation. Additionally, other factors such as protein aggregation exhibited inhibitory effects on oxidative modification.

Key words: cytochrome c, oxidative modification, Girard’s reagent T, methylation, NMR

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