天眷波谱青年论坛特邀稿件

一种示踪干细胞的磁共振-荧光双模成像探针

  • 赵敏敏 ,
  • 张艳辉 ,
  • 张朋利 ,
  • 谭波 ,
  • 蒋海珍 ,
  • 张海禄 ,
  • 邓宗武
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  • 1. 上海大学 理学院, 上海 200444;
    2. 中国科学院 苏州纳米技术与纳米仿生研究所, 江苏 苏州 215123
张海禄博士,中国科学院苏州纳米技术与纳米仿生研究所研究员,2008年博士毕业于中国科学院武汉物理与数学研究所.目前任江苏省核磁共振专业委员会副主任委员,中华放射学会磁共振物理与工程学组委员,中国晶体学会药物学专业委员会常务委员.他的研究领域主要为药物固态化学和纳米-生物界面物理.

收稿日期: 2018-06-06

  网络出版日期: 2018-07-31

基金资助

the National Natural Science Foundation of China (31371010, 21673281); Funds from Youth Innovation Promotion Association of the Chinese Academy of Sciences (2012242); Key Research Project from the Ministry of Science and Technology of China (2017YFA0104300).

A Magnetic Resonance-Fluorescent Dual-Mode Imaging Probe for Stem Cell Tracking

  • ZHAO Min-min ,
  • ZHANG Yan-hui ,
  • ZHANG Peng-li ,
  • TAN Bo ,
  • JIANG Hai-zhen ,
  • ZHANG Hai-lu ,
  • DENG Zong-wu
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  • 1. College of Sciences, Shanghai University, Shanghai 200444, China;
    2. Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215123, China

Received date: 2018-06-06

  Online published: 2018-07-31

Supported by

the National Natural Science Foundation of China (31371010, 21673281); Funds from Youth Innovation Promotion Association of the Chinese Academy of Sciences (2012242); Key Research Project from the Ministry of Science and Technology of China (2017YFA0104300).

摘要

本文设计并合成了Gd基磁共振-荧光双模成像探针——Gd-DOTA-PEG-GA,通过电穿孔的方式标记人源间充质干细胞(hMSCs).电穿孔标记诱导细胞将探针组装成团簇状纳米粒子进入细胞质,显著延长其与细胞结合的时间,并呈现出明显的T2信号减弱效应,且信号减弱效应可以持续7天以上.在水溶液中,该探针的发射带集中在498 nm,并且荧光强度在一周内无明显衰减.该探针标记的细胞在荧光倒置显微镜下呈现绿色荧光.这些结果表明该探针可以作为磁共振-荧光双模成像探针用于干细胞示踪.

本文引用格式

赵敏敏 , 张艳辉 , 张朋利 , 谭波 , 蒋海珍 , 张海禄 , 邓宗武 . 一种示踪干细胞的磁共振-荧光双模成像探针[J]. 波谱学杂志, 2019 , 36(2) : 127 -137 . DOI: 10.11938/cjmr20182659

Abstract

A magnetic resonance (MR)-fluorescent dual-mode imaging probe was prepared by conjugating guaiazulene to Gd-DOTA, and used to label human mesenchymal stem cells (hMSCs) via electroporation. The probe self-assembled into nanoclusters in the cytoplasm, resulting in a significant reduction in T2-weighted signal intensity that persisted up to 7 days. The fluorescence signal of the probe was observed at 498 nm, and the labelled hMSCs emitted a green fluorescence. The MR-fluorescent dual-mode imaging probe can potentially be used for stem cell tracking in vivo.

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