1H MRS评价长期胰岛素治疗1型糖尿病大鼠海马代谢物的变化
收稿日期: 2021-11-04
网络出版日期: 2021-12-31
基金资助
江汉大学校级科研项目资助计划(2022SXZX26);国家自然科学基金资助项目(81601204)
Hippocampal Metabolite Alterations in Long-term Insulin-treated Type 1 Diabetes Mellitus Rats Revealed by 1H MRS
Received date: 2021-11-04
Online published: 2021-12-31
本研究应用质子磁共振波谱（1H MRS）技术对链脲佐菌素（STZ）诱导的1型糖尿病（T1DM）大鼠及长期胰岛素治疗的T1DM大鼠单侧海马的代谢物进行了分析. 结果发现，与对照组大鼠及胰岛素治疗组大鼠相比，T1DM模型组大鼠空腹血糖显著升高，体重显著降低（p < 0.05）.T1DM模型组肌醇（Ins）、牛磺酸（Tau）与谷氨酸（Glu）浓度较对照组显著升高（p = 0.000、p = 0.003、p = 0.014）.胰岛素治疗组Ins与Tau浓度较T1DM模型组显著降低（p = 0.000、p = 0.010），与对照组无差别；而Glu、谷氨酸和谷氨酰胺（Glx）浓度较对照组显著升高（p = 0.007、p = 0.042）.本文结果表明T1DM大鼠海马区代谢物Ins浓度与Tau浓度对胰岛素治疗敏感.
徐慧 , 吴伊婷 , 王旭霞 , 康彦 , 雷皓 , 高丽凤 . 1H MRS评价长期胰岛素治疗1型糖尿病大鼠海马代谢物的变化[J]. 波谱学杂志, 2022 , 39(4) : 393 -400 . DOI: 10.11938/cjmr20212955
In this study, 1H magnetic resonance spectroscopy (1H MRS) was used to analyze metabolites in the hippocampus of chronic type 1 diabetes mellitus (T1DM) rats induced by streptozocin and T1DM rats treated with long-term insulin. The results showed that, compared with control rats and insulin-treated T1DM rats, the fasting blood glucose of T1DM rats increased significantly, while the body weight of T1DM rats decreased remarkably (p < 0.05). The contents of myo-inositol (Ins), taurine (Tau) and glutamate (Glu) of T1DM rats were significantly higher than those of control rats (p=0.000, p=0.003, p=0.014, respectively), and the contents of Ins and Tau of insulin-treated T1DM rats were significantly decreased than those in the T1DM diabetic rats (p=0.000, p=0.010, respectively). Contents of Glu and Glx (Glu and glutamine) of insulin-treated T1DM rats were significantly higher than those in the control group (p=0.007, p=0.042, respectively). The metabolite changes of Ins and Tau in hippocampus of T1DM rats are sensitive to insulin treatment.
Key words： 1H MRS; metabolite; hippocampus; type 1 diabetes mellitus; insulin
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