基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪
收稿日期: 2022-03-21
网络出版日期: 2022-04-28
基金资助
国家重点基础研究发展计划资助项目(2017YFA0505400);国家重点基础研究发展计划资助项目(2018YFE0202300);国家重点基础研究发展计划资助项目(2018YFA0704002)
Track the Conformational Change of Unlabeled Yeast Cytochrome c in Cell Homogenate Using NMR
Received date: 2022-03-21
Online published: 2022-04-28
细胞色素c（cytochrome c，cyt c）是一个重要的多功能蛋白. 在线粒体中，它作为载体传递电子. 而在细胞质中，它可能会作为凋亡起始因子启动细胞凋亡程序. 复杂的细胞质环境是否会对其构象产生影响，以及产生怎样的影响，目前仍然没有得到确证. 本研究通过无标记的基于甲基的核磁共振（nuclear magnetic resonance，NMR）技术追踪了野生型酿酒酵母iso-1 cyt c在酵母细胞匀浆液中的构象变化. 发现cyt c在细胞匀浆中至少存在4种不同的氧化态构象和1种还原态构象. 而且随着时间的推进，不同构象之间发生转换. 结果表明cyt c的构象会随环境改变，这可能与抵抗氧化应激相关.
占建华 , 胡琴 , 朱勤俊 , 蒋滨 , 张许 , 刘买利 . 基于磁共振的胞浆中无标记酵母细胞色素c构象变化追踪[J]. 波谱学杂志, 2023 , 40(1) : 22 -29 . DOI: 10.11938/cjmr20222985
Cytochrome c (cyt c) is an important multifunctional protein. In mitochondria, it acts as a carrier for electron transporting. In cytoplasm, it may act as an apoptotic initiator to initiate the apoptotic process. Whether and how a complex cytoplasmic environment affects its conformation has not been confirmed yet. In this study, the conformational changes of wild type saccharomyces cerevisiae iso-1 cyt c in yeast cell homogenate were tracked by methyl-based nuclear magnetic resonance (NMR) technique. At least four different oxidative conformations and one reduced conformation of cyt c were identified in cell homogenate. And over time, the transitions among different conformations of cyt c were observed. The results indicate that the conformation of cyt c changes with environment, which may be closely related to resistance to oxidative stress.
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