氟醚菌酰胺是一种广谱类杀菌剂，联合使用高分辨率质谱（HR-MS）、紫外光谱（UV）、红外光谱（IR）及核磁共振波谱（NMR）对氟醚菌酰胺的结构进行了鉴定，指出了紫外光谱的特征吸收带、红外光谱的特征吸收峰及其对应的各官能团的振动形式，同时对核磁共振氢谱（¹H NMR）、碳谱（¹³C NMR）、无畸变极化转移增强谱（DEPT）、同核位移相关谱（¹H-¹H COSY）、异核单量子相关谱（¹H-¹³C HSQC）、异核多键相关谱（¹H-¹³C HBMC）进行了解析，指认了相关基团的连接顺序，确证了该化合物的结构．波谱学信息及化合物结构的确证不仅对农药生产过程中的质量管控有着非常重要的意义，还为氟醚菌酰胺类似物的合成提供参考．

Fluopimomide is a broad-spectrum fungicide whose structure was characterized using high-resolution mass spectrometry (HR-MS), ultraviolet spectroscopy (UV), infrared spectroscopy (IR), and nuclear magnetic resonance spectroscopy (NMR). The characteristic absorption bands in the UV spectrum, as well as the characteristic absorption peaks in the IR spectrum and their corresponding vibrational modes of functional groups were identified. Furthermore, the structure was confirmed through comprehensive analysis of ¹H NMR spectrum, ¹³C NMR spectrum, distortionless enhancement by polarization transfer (DEPT), homonuclear correlation spectrum (¹H-¹H COSY), heteronuclear single quantum coherence spectroscopy (¹H-¹³C HSQC), and heteronuclear multiple bond correlation spectroscopy (¹H-¹³C HMBC). The connectivity of relevant groups was assigned, thereby unequivocally verifying the compound's structure. The spectroscopic data and structural confirmation are crucial for quality control during pesticide production and provide a valuable reference for the synthesis of fluopimomide analogs.