绿豆(Vigna radiata) 代谢物组成的核磁共振定量分析
收稿日期: 2014-01-08
修回日期: 2014-11-06
网络出版日期: 2014-12-05
基金资助
国家自然科学基金资助项目(21175149, 21221064).
Quantitative Analysis of Metabolites in Mungbean (Vigna Radiata) Extracts using NMR Techniques
Received date: 2014-01-08
Revised date: 2014-11-06
Online published: 2014-12-05
Supported by
国家自然科学基金资助项目(21175149, 21221064).
吴香玉1 , 2 , 李 宁1 , 2 , 唐惠儒1* . 绿豆(Vigna radiata) 代谢物组成的核磁共振定量分析[J]. 波谱学杂志, 2014 , 31(4) : 548 -563 . DOI: 10.11938/cjmr20140409
Mungbean (Vigna radiata) is an important crop that has various beneficial functions such as antioxidant and anti-inflammatory activities. However, the metabolites in mungbean have only been partially identified and quantified in previous studies. In this study, we systematically identified and assigned 48 metabolites in mungbean (Elü No.4) seeds with J-RES, COSY, TOCSY, HSQC, HMBC and 2D NMR spectra. In particular, the composition of raffinose family oligosaccharides in the extracts was determined using DOSY experiments. Most of the metabolites in the extracts were quantified. This study will provide useful information for mungbean metabolomic and nutritional studies.
Key words： nuclear magnetic resonance; assignment; 2D NMR; mungbean; quantification
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