磷脂酰胆碱SUV 的NMR 研究
收稿日期: 2014-04-24
修回日期: 2014-10-28
网络出版日期: 2014-12-05
基金资助
国家自然科学基金资助项目(21075132)，国家重点基础研究发展计划(“973 计划”)资助项目(2013CB910200).
An NMR Study of Phosphatidylcholine Small Unilamellar Vesicles
Received date: 2014-04-24
Revised date: 2014-10-28
Online published: 2014-12-05
Supported by
国家自然科学基金资助项目(21075132)，国家重点基础研究发展计划(“973 计划”)资助项目(2013CB910200).
磷脂酰胆碱（PC）是人和动物血液中大量存在的一类具有重要生物功能的磷脂，它和鞘磷脂一起形成了不同大小的密度脂蛋白，对血液中胆固醇等分子的转运和代谢起着至关重要的作用．脂蛋白中磷脂的组成和形态变化与某些疾病，如动脉粥样硬化、癌症和老年痴呆等的发生和发展密切相关，因此研究磷脂的组成形态将有助于明确磷脂的生物化学作用．该文采用一维(1D)和二维(2D) NMR 技术对PC 所形成的SUV (small unilamellar vesicle)结构进行了分析，通过对PC 磷脂头部氮甲基的检测分析，发现PC 所形成的SUV为较为稳定的双层结构，这表明通常的磷脂脂蛋白可能是一种双层膜结构，而非通常所认为的单层结构．
李楠1 , 2 , 孙鹏1 , 2 , 刘买利1 , 张许1* . 磷脂酰胆碱SUV 的NMR 研究[J]. 波谱学杂志, 2014 , 31(4) : 572 -578 . DOI: 10.11938/cjmr20140411
Phosphatidylcholines (PC) is one of the most important phospholipids in the human and animal serum. It is the major constituent of lipoproteins that play important roles in the transport and metabolism of cholesterol. It was found that the many diseases, such as atherosclerosis, cancer, dementia and so on, were often associated with compositional and structural changes of phospholipids. Therefore, it is of interest to study the composition and structure of phospholipids. Here, small unilamellar vesicles (SUV) formed by PC were studied using 1D and 2D NMR experiments. It was found that there were two different [-N+(CH3)3] signals of PC, indicating that the SUV formed by PC may have a bilayer-spherical structure, not as previously thought to be a mono-layer structure.
Key words： NMR; bilayers; small unilamellar vesicles; phosphotidylcholine
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