一种用于 fMRI 的嗅觉刺激装置设计与验证
收稿日期: 2013-11-11
修回日期: 2014-10-28
网络出版日期: 2014-12-05
基金资助
中科院科研装备研制资助项目(YZ201253)，苏州市科技资助项目(SYG201125).
Design and Evaluation of an Olfactometer for fMRI Studies
Received date: 2013-11-11
Revised date: 2014-10-28
Online published: 2014-12-05
Supported by
中科院科研装备研制资助项目(YZ201253)，苏州市科技资助项目(SYG201125).
为了实现任务态fMRI 的嗅觉刺激装置的设计和功能验证．满足嗅觉fMRI 实验的自动化刺激要求．首先，针对嗅觉刺激和人体嗅觉感受的特点，结合使用环境的要求，总结嗅觉fMRI 的实验设计方法，归纳出用于fMRI 的嗅觉刺激装置的具体需求．将刺激装置分为气路系统和控制系统、气路系统采用洁净空气通过气味溶液产生相应的气味气体，通过多支路切换达到输出不同气味的目的；控制系统采用虚拟仪器方案，其软件基于LabVIEW 平台编程，提供可输入刺激序列的人机界面，并根据要求控制电磁阀切换不同气路．然后，使用该刺激装置对单个被试进行嗅觉刺激并成像，刺激气体采用乙酸异戊酯和吡啶，实验为组块设计，使用Siemens 3.0 T MRI 仪器EPI 序列进行扫描，数据处理采用基于Matlab 软件的SPM8 和MarsBaR 工具包．选取眶额叶和岛叶中的脑激活团簇作为感兴趣区域(ROI)进行分析，激活信号在时间上的强度变化与刺激序列变化基本一致，吡啶组块的激活信号强度大于乙酸异戊酯组块激活强度，且其强度变化比乙酸异戊酯组块更符合刺激序列．ROI 分析证明刺激装置基本满足嗅觉fMRI 实验需要，具有较强的可用性．此外，在相同的刺激时间和间隔时间下，人脑同一区域对不同刺激气味的激活反应可能不同．
关键词： 功能磁共振成像(fMRI); 刺激装置; 嗅觉; 刺激
王骁冠1–3 , 杨晓冬3* , 王慧3 , 常严3 , 徐雅洁3 , 张广才3 , 蒋瑞瑞1–3 . 一种用于 fMRI 的嗅觉刺激装置设计与验证[J]. 波谱学杂志, 2014 , 31(4) : 477 -487 . DOI: 10.11938/cjmr20140403
We developed a computer-controlled olfactometer for delivering odors for functional magnetic resonance imaging (fMRI) experiments. The olfactometer is consisted of an air transport system and a control system. Odors are produced and delivered by the air transport system, in which air flow first passes through liquid mixed with odorants in gas-washing bottles and then is delivered to the test subjects. The control system provides a user interface for
operating the device and controlling solenoid valves. The system is based on virtual instrumentation technology, and the software for it is written in LabVIEW. The device was tested on a Siemens 3.0 T MRI scanner. Block-design fMRI data were collected from one healthy subject who was stimulated with two odors, isoamyl acetate and pyridine. The raw fMRI data were processed with Matlab-based SPM8 and MarsBaR toolbox. Region-of-interest (ROI)-based analysis was performed for the left orbitofrontal cortex and left insula. It was found that the signal changes in the two ROIs were basically in compliance with the stimulation paradigm, but the signal changes induced by pyridine were more robust than that produced by isoamyl acetate stimulation. In the same ROI, the signal intensity changes caused by pyridine were higher than that induced by isoamyl acetate.
Key words： fMRI; olfactometer; olfactory; stimulation
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