牡丹不同根部位的代谢物分布

肖超妮; 王培; 马翠霞; 荣泽铭; 郑晓晖*

doi:10.11938/cjmr20150410

波谱学杂志 >

2015 , Vol. 32 >Issue 4: 648 - 660

DOI: https://doi.org/10.11938/cjmr20150410

研究论文

牡丹不同根部位的代谢物分布

肖超妮 ,
王培 ,
马翠霞 ,
荣泽铭 ,
郑晓晖*

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西北大学生命科学学院，陕西西安 710069

肖超妮(1975-)，女，陕西户县人，博士，主要从事中药学研究． *通讯联系人：郑晓晖，电话： 029-88302686， E-mail： zhengxh@nwu.edu.cn.

收稿日期: 2015-10-16

修回日期: 2015-11-09

网络出版日期: 2015-12-05

基金资助

陕西省重点科技创新团队资助项目(2013KCT-24)

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The Distribution of Metabolites in Different Root Parts from Tree Peony

XIAO Chao-ni ,
WANG Pei ,
MA Cui-xia ,
RONG Ze-ming ,
ZHENG Xiao-hui*

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College of Life Sciences, Northwest University, Xi’an 710069, China

*Corresponding author： ZHENG Xiao-hui, Tel: +86-29-88302686, E-mail: zhengxh@nwu.edu.cn.

Received date: 2015-10-16

Revised date: 2015-11-09

Online published: 2015-12-05

Supported by

陕西省重点科技创新团队资助项目(2013KCT-24)

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摘要

该文应用核磁共振（ NMR）和高效液相色谱-质谱（ HPLC-MS）技术系统分析了牡丹不同根部位的代谢物组成，共鉴定了包括蔗糖、苯乙酰类（ 4 种）、酚酸类（ 6 种）、单萜苷类（ 2 种）、黄酮类（ 1 种）和不饱和脂肪酸（ 2 种）共 16 种代谢物．在牡丹根中，丹皮酚以及丹皮酚苷等苯乙酰类成分和蔗糖的含量较高，而酚酸、黄酮和单萜苷类成分的含量较低．基于 NMR 的代谢组分析表明，牡丹不同根部位的代谢物组成具有明显差异：在二级侧根、一级侧根和主根中，丹皮酚及丹皮酚苷类的含量依次较高而蔗糖含量依次降低，这可能与不同根部位的植物生理有关；和侧根相比，主根中具有活性的代谢物含量较高，推测主根的品质优于侧根，这为市场上牡丹根皮的等级划分提供了重要的科学依据．

关键词： 核磁共振（ NMR）; 高效液相色谱-质谱（ HPLC-MS）; 代谢物; 根部位; 牡丹

本文引用格式

肖超妮 , 王培 , 马翠霞 , 荣泽铭 , 郑晓晖* . 牡丹不同根部位的代谢物分布[J]. 波谱学杂志, 2015 , 32(4) : 648 -660 . DOI: 10.11938/cjmr20150410

Abstract

The distribution of metabolites in the root bark of tree peony from different root parts was systematically analyzed using the combination of NMR and HPLC-MS for the first time. A total of 16 metabolites from their methanol extracts were simultaneously identified and quantified, including 1 primary metabolite (sucrose) and 15 secondary ones such as 4 acetophenones, 6 phenolics, 2 monoterpene glycosides, 1 flavonoid and 2 unsaturated fatty acids. The quantitative data indicated that there are the higher levels of acetophenones and sucrose, and the lower contents of phenolics, monoterpene glycosides, flavonoids and unsaturated fatty acids in the tree peony roots. NMR-based metabolomics revealed that the metabolite compositions were found to be different among the three root parts. The increasing levels of acetophenones and decreasing level of sucrose were observed in second lateral root, the first lateral and axial root in order. The axial roots have higher quality than the lateral roots in tree peony due to the accumulation of bioactive secondary metabolites associated with plant physiology. These findings provided important scientific evidence for grading the root bark of tree peony on the general market.

Key words： NMR; HPLC-MS; metabolite; root part; tree peony

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