棕色脂肪组织和白色脂肪组织的代谢组学研究
收稿日期: 2015-05-14
修回日期: 2016-04-09
网络出版日期: 2016-06-05
基金资助
国家自然科学基金资助项目(91439102).
Metabonomics Analysis of Brown Adipose and White Adipose Tissues
Received date: 2015-05-14
Revised date: 2016-04-09
Online published: 2016-06-05
棕色脂肪组织(brown adipose tissue, BAT)在机体的能量代谢中起着极其重要的作用,且被认为是治疗肥胖的潜在靶点之一,然而目前对于BAT功能的代谢基础并不清楚.该文使用了基于核磁共振(NMR)和气相色谱(GC)技术的代谢组学方法,描述和比较了BAT与白色脂肪组织(white adipose tissue, WAT)的水溶性代谢物和脂肪酸组成的差异.研究结果表明,两种脂肪组织在糖代谢、氨基酸代谢、脂肪酸代谢、核苷酸代谢、胆碱代谢等多种代谢通路上均具有显著性差异,且这些差异与两种组织不同的生物学作用密切相关.以上研究结果将为解析BAT功能分子机制提供了线索和基础数据.
关键词： 核磁共振(NMR); 气相色谱(GC); 代谢组学; 脂肪酸; 棕色脂肪组织(BAT); 白色脂肪组织(WAT)
宋懿朋 , 李宁 , 李晶 , 王玉兰 , 薛海斯 . 棕色脂肪组织和白色脂肪组织的代谢组学研究[J]. 波谱学杂志, 2016 , 33(2) : 208 -223 . DOI: 10.11938/cjmr20160204
Brown adipose tissue (BAT) plays a vital role in energy metabolism, and has been regarded as a novel potential drug target for treating obesity. However, the metabolic compositions of BAT and white adipose tissue (WAT) remain to be determined. We applied nuclear magnetic resonance (NMR) and gas chromatography (GC) based metabonomics approaches to characterize and compare the composition of water-soluble metabolites and fatty acids in BAT and WAT. The results indicated that these two types of adipose tissue have marked differences in carbohydrate metabolism, amino acid metabolism, fatty acids metabolism, nucleotide metabolism and choline metabolism pathways, and these differences are tightly associated with their unique biological functions. Our research provided clues and basic information for elucidation of the function of BAT at molecular level.
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