Abstract: In this article, ¹H and ¹³C NMR spectra of Gestodene were analyzed by using two-dimensional ¹H-¹H COSY, heteronuclear ¹H-¹³C shift correlation and HMBC, It was made possible to assign ¹H and ¹³C NMR spectra of Gestodene completely by combination of these techniques, Gestodene showed complex ¹H signal around 1.4-2.6ppm and ¹³C signals around 25.3-38.13ppm, This combination of techniques is applicable for assignment of highly overlapping signals, The HMBC spectra can provide valuable information for assignment of ¹H and ¹³C NMR signals.

Key words: Gestodene, Oral contraceptive, 2D NMR, Assignment of spectral lines