Abstract: The alkaloids were isolated from the bulb of Fritillaria wabuensis S.Y.Tang et S.C. Yueh the best "Chuan Bei" cultivated in Sichuan Province, the structure and conformation of an alkaloid was studied and determined as imperialine-β-N-Oxide by ¹H-¹HCOSY、¹³C-¹HCOSY MMR 2D-nuclear magnetic resonance technique, In this paper, we report the detailed research of very rare imperialine-β-N-Oxides in nature using NOESY,HMQC and HMBC 2D NMR new technique.

Key words: Fritillaria wabuense, Imperialine-β-N-Oxide, 2D NMR