2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES

Chinese Journal of Magnetic Resonance ›› 1994, Vol. 11 ›› Issue (2): 219-222.

2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES

Zhang Yibing, Yu Yihua, Shen Wei, Wang Qiwen

State Key Laboratory or Bio-organic and Natural products Chemistry, Shanghai Institute of Organic Chemistry, Chinese Academy of Sciences, Shanghai 200032

Received:1993-01-04 Revised:1993-04-20 Published:1994-06-05 Online:2018-01-20

Abstract

Abstract: The main difficulty in 2D NOESY of mcdium-sized peptdes is lacking of cross-peaks, espccially in the region related with NH, For solving this problem, these authors use a mixed solvent,CD₃OD/H₂O, to dissolve peptides,which allows NMR measurements at low temperature.The increased viscosity of solution and the correlation times by low-temperature measurements made a lot of intra-and inter-residue NOES observable, which helped us unambiguously complete the assigmnents of a nonapeptide and an undccapcptide, and further in calculation of H-Hdistances.

Key words: 2D NOESY, Medium-sized peptides

Zhang Yibing, Yu Yihua, Shen Wei, Wang Qiwen. 2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES[J]. Chinese Journal of Magnetic Resonance, 1994, 11(2): 219-222.

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[3]	Miao Xijia, Mao Shizhen, Lin Donghai, Ye Chaohui. DETERMINATION OF INTERNUCLEAR DISTANCES FROM THE NONSYMMETRIC 2D NOESY METHOD Ⅲ. Extraction of the Complete 2D NOESY Mixing Coefficient Matrix from the Nonsymmetric 2D NOESY Overlapping Peak Intensities [J]. Chinese Journal of Magnetic Resonance, 1995, 12(3): 261-268.
[4]	Miao Xijia, Han Xiuwen, Hu Jiehan. DKTERMINATION OF INTERNUCLEAR DISTANCES FROM THE NONSYMMETRIC 2D NOESY METHOD 2.The Nonsymmetric 2D NOESY Experiment for Cis-Pt(NH₃)₂(Guo)₂Cl₂ [J]. Chinese Journal of Magnetic Resonance, 1994, 11(1): 27-33.
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[6]	Miao Xijia, Han Xiuwen, Hu Jiehan. DETERMINATION OF INTERNUCLEAR DISTANCES FROM THE NONSYMMETRIC 2D NOESY METHOD——1. Measurement for the full matrix of,2D NOESY mixing coefficient by the nonsymmetric, 2D NOESY [J]. Chinese Journal of Magnetic Resonance, 1993, 10(3): 241-250.

2D NOESY EXPERIMENTAL PROBLEMS OF MEDIUM-SIZED PEPTIDES

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