Structure Elucidation of Hexpolyamide/Ser-His Dipeptide Conjugates by NMR and ESI-MS/MS

Chinese Journal of Magnetic Resonance ›› 2006, Vol. 23 ›› Issue (4): 509-515.

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Structure Elucidation of Hexpolyamide/Ser-His Dipeptide Conjugates by NMR and ESI-MS/MS

YE Yong¹; HU Xiao-lian¹; ZHANG Chang-shui¹; SHEN Li-li¹; CAO Li-feng¹; NIU Ming-yu¹; LIAO Xin-cheng¹*; ZHAO Yu-fen^1,2

(1.Department of Chemistry, Key Laboratory of Chemical Biology and Organic Chemistry, Zhengzhou University, Zhengzhou 450052, China; 2.The Key laboratory for Bioorganic Phosphorus Chemistry & Chemical Biology, Ministry of Education, Department of Chemistry, Tsinghua University, Beijing 100084, China)

Received:2006-03-16 Revised:2006-04-20 Published:2006-12-05 Online:2009-12-05
Contact: LIAO Xin-cheng

Abstract

Abstract: A hexpolyamide/seryl-histidine dipeptide conjugate DNA cleavage agent was synthesized and its structure was elucidated by NMR and ESIMS/MS methods. Complete assignment of ¹H and ¹³C NMR chemical shifts of the compound was obtained using 2D NMR techniques, including ¹H-¹H COSY, HSQC and HMBC. The MS/MS fragmentation pathways of the polyamide/peptide conjugate are also discussed.

Key words: NMR, chemical shift, ESI-MS/MS, hexpolyamide, seryl-histidine dipeptide, conjugate

CLC Number:

O641

YE Yong¹; HU Xiao-lian¹; ZHANG Chang-shui¹; SHEN Li-li¹; CAO Li-feng¹; NIU Ming-yu¹; LIAO Xin-cheng¹*; ZHAO Yu-fen^1,2
. Structure Elucidation of Hexpolyamide/Ser-His Dipeptide Conjugates by NMR and ESI-MS/MS
[J]. Chinese Journal of Magnetic Resonance, 2006, 23(4): 509-515.

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Structure Elucidation of Hexpolyamide/Ser-His Dipeptide Conjugates by NMR and ESI-MS/MS

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