Chinese Journal of Magnetic Resonance ›› 2006, Vol. 23 ›› Issue (1): 1-10.

• Articles •     Next Articles

Assignment of 1H and 15N Chemical Shifts and Structure Elucidation of C-terminal FF-domain of the Formin-Binding Protein

  

  1. Key Laboratory of Optical and Magnetic Reasonance Spectroscopy for Education Ministry, Physics Department of East China Normal University, Shanghai 200062, China
  • Received:2005-06-07 Revised:2005-10-17 Published:2006-03-05 Online:2006-03-05

Abstract:

FF-domains have been found widely in many signaling proteins. In this paper, the structure characteristics of FF-domain of the formin-binding protein 11 (FBP11) were studied by NMR spectroscopy. The spin systems for most of the amino acid residues in the FF-domain were identified by analyzing NMR spectra acquired in H2O and D2O. Sequence specific assignment of the spin systems was made using dαN, dNN and dβN NOEs correlation calculated from NOESY spectra. The secondary structure of the FF-domain was found to include three α helices.

Key words: NMR spectroscopy, FF-domain, chemical shift assignment, secondary structure, formin-binding protein

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