Chinese Journal of Magnetic Resonance

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THE 1H NMR RESONANCE ASSIGNMENT OF PEPTIDE mHCN1 WITH WET SOLVENT SUPPRESSION METHOD

SHAO Rong-rong, SUI Tao, SONG Guo-qiang*   

  1. Institute of Materia Medica,Shanghai Institute of Biological Science,Chinese Academy of Sciences,Shanghai 200031, China
  • Published:2002-03-05 Online:2002-03-05
  • About author:*Correspondence author

Abstract:

Like K+ channel, the pore region of HCN channel includes GYG characteristic se quence. But besides K+, Na+ can selectively overpass HCN channel. The conf ormation study of mHCN1 pore region peptide helps to understand the selectivity  of HCN channel. In this article, new developed WET sequence was used to suppress  solvent peak. With the comprehensive use of wet-TOCSY and wet-NOESY NMR exper iments, the 1H resonance of mHCN1 pore region peptide was assigned.(95%H2O+5%D2O).

Key words: mHCN1, WET, solvent suppression method,  1H reso nance assignment

CLC Number: