To investigate the feasibility of serum ³¹P NMRS in the study phospholipid metabolites and diagnosis of leukaemia. Method: Serum sample of leukaem

ia patients and healthy controls were collected and measured by ³¹P NMRS with MSL-300MHz spectrometer. The phospholipid contents were known by contraxt relatively integral area of PC signal and (PE+SM) signal. Results: In spectra of serum of leukaemia patients, the phospholipid signal intensities were significantly decreases. It reflected lower phosphatidylcholine and phosphatidylcholine and sphingomyelin content in serum of leukemia patients, especially for original untreatment patient. Conclusions: These results suggest that serum ³¹P NMRS may likely become a promising new method for the study of phospholipid changes or metabolites and identifying the presence of leukaemia.