Abstract:

Hongguanggenin and two hongguanggenin-based saponins, Cantalasaponin-1 and  Ag ameroside-1 were isolated from fermented leaf of Agave americana L. recentl y. On  the basis of 2D NMR techniques, including DQF ¹H-¹H COSY，HSQC，HMBC，H SQC-TOCSY，ROESY and so on, NMR complete assignments of  the three compounds and the glyc osylation shift effect of hongguanggenin were obtained. This led to the revision of some carbon assignments and glycoylation shift effect in the reference.

Key words: Hongguanggenin, Cantalasaponin-1, Agameroside-1,  Agave americana L, NMR complete assignments, Glycosylation shift effect