Chinese Journal of Magnetic Resonance >

2017 , Vol. 34 >Issue 3: 266 - 274

DOI: https://doi.org/10.11938/cjmr20162527

Effects of Metal Ions on Human Serum Albumin Studied by Radiation Damping Water-Ligand Observed via Gradient Spectroscopy

  • CHEN Yao ,
  • SUN Peng ,
  • LIU Mai-li ,
  • ZHANG Xu
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  • 1. State Key Laboratory of Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan(Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences), Wuhan 430071, China;
    2. University of Chinese Academy of Sciences, Beijing 100049, China

Received date: 2016-05-10

  Revised date: 2017-07-18

  Online published: 2017-09-05

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Abstract

Human serum albumin (HSA) contains many metabolite binding sites. It has been widely studied for its functions in drug transportation. Nuclear magnetic resonance (NMR) is a frequently used tool to study HSA. However, due to its high molecular weight, the NMR spectra of HSA acquired with the conventional methods are often crowded, making spectral assignment and data interpretation difficult. In this study, a new method, which combined radiation damping water-ligand observed via gradient spectroscopy (RD-WaterLOGSY) with transverse relaxation weighted (T2W) techniques, was proposed to simplify the NMR spectra of HSA. With the T2W-RD-WaterLOGSY technique, the effect of pH on HSA was studied, as well as the interactions between HSA and Zn2+. The results showed that the relative changes in chemical shift could be used as a probe to analyze the pH changes in HSA solution and the interactions between HSA and Zn2+.

Key words： NMR; human serum albumin (HSA); Zn2+; interaction; T2W-RD-WaterLOGSY

Cite this article

CHEN Yao , SUN Peng , LIU Mai-li , ZHANG Xu . Effects of Metal Ions on Human Serum Albumin Studied by Radiation Damping Water-Ligand Observed via Gradient Spectroscopy[J]. Chinese Journal of Magnetic Resonance, 2017 , 34(3) : 266 -274 . DOI: 10.11938/cjmr20162527

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