Review Articles

Probing Membrane Protein Interactions by 19F Solid-State NMR

  • XU Xiao-jun ,
  • WANG Shen-lin
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  • 1. College of Chemistry and Molecular Engineering, Peking University, Beijing 100871, China;
    2. Beijing NMR Center, Peking University, Beijing 100871, China;
    3. National Laboratories of Beijing National Laboratory for Molecular Science, Beijing 100871, China

Received date: 2018-03-12

  Online published: 2018-05-03

Supported by

the National Key Research and Development Program of the Ministry of Science and Technology, People's Republic of China (2016YFA0501203); the National Natural Science Foundation of China (31470727); the interdisciplinary medicine Seed Fund of Peking University; the 1000 Plan for Young Talent Program of China.

Abstract

The 19F nucleus has a high gyromagnetic ratio that is comparable to that of proton. Due to its high sensitivity and low background in the biological systems, 19F labeling has emerged as an attractive approach in biological solid-state NMR studies. In this review, we summarized the widely used 19F-labeling strategies, 19F solid-state NMR spectroscopy techniques to probe membrane protein interactions, and the recent applications of 19F solid-state NMR in characterizing of membrane proteins. The limitations of 19F solid-state NMR were also highlighted.

Cite this article

XU Xiao-jun , WANG Shen-lin . Probing Membrane Protein Interactions by 19F Solid-State NMR[J]. Chinese Journal of Magnetic Resonance, 2019 , 36(2) : 238 -251 . DOI: 10.11938/cjmr20182618

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