Chinese Journal of Magnetic Resonance >
Cathepsin B Triggered Hyperpolarization 129Xe MRI Probe for Ultra-Sensitive Lung Cancer Cells Detection
Received date: 2020-04-25
Online published: 2020-05-27
Cathepsin B (Cat B) is a lysosomal cysteine protease that plays an essential role in cellular metabolism. Cat B is overexpressed in lung cancer cells. Fluorescence imaging techniques have been developed for measuring intracellular Cat B levels, which, however, suffers from limited penetration depth and interferences from autofluorescence background. To resolve these technical difficulties, we designed a novel Cat B biosensor detected by hyperpolarized xenon magnetic resonance imaging. It consists of a cryptophane cage as a 129Xe NMR reporter and an amide bond as a Cat B-specific cleavable group. When the biosensor interacts with Cat B, the cleavage of amide bond leads to changes in 129Xe chemical shift. Combing with hyperpolarization-chemical exchange saturation transfer (Hyper-CEST), our biosensor provides a novel method for responsive detection of Cat B.
Chong-wu WANG , Xi HUANG , Lei SHI , Shi-zhen CHEN , Xin ZHOU . Cathepsin B Triggered Hyperpolarization 129Xe MRI Probe for Ultra-Sensitive Lung Cancer Cells Detection[J]. Chinese Journal of Magnetic Resonance, 2021 , 38(3) : 336 -344 . DOI: 10.11938/cjmr20202828
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