Chinese Journal of Magnetic Resonance >

2021 , Vol. 38 >Issue 2: 155 - 163

DOI: https://doi.org/10.11938/cjmr20202854

Articles

An NMR Study on the clpC Operon Binding Region of Transcription Factor CtsR from Bacillus subtilis

  • Xiao-wen CHEN ,
  • Bi-ling HUANG ,
  • Shao-hua HUANG ,
  • Yu-fen ZHAO
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  • 1. Institute of Drug Discovery Technology, Ningbo University, Ningbo 315211, China
    2. Department of Chemical Biology, College of Chemistry and Chemical Engineering, and the Key Laboratory for Chemical Biology of Fujian Province, Xiamen University, Xiamen 361005, China
    3. Key Lab of Bioorganic Phosphorus Chemistry & Chemical Biology, Department of Chemistry, Tsinghua University, Beijing 100084, China

Received date: 2020-09-21

  Online published: 2020-11-20

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Abstract

When Gram-positive bacterium Bacillus subtilis responds to heat-shock, protein arginine kinase McsB phosphorylates Arg62 (R) in the clpC operon binding region of transcription factor CtsR, leading to dissociation of CtsR and clpC operon and initiation of stress-genes transcription for the ensuing expression of heat-shock proteins. This work investigated the clpC operon binding region (KRGGGG) of CtsR with NMR spectra (i.e., 1H NMR, 1H-1H COSY, 1H-1H TOCSY, 1H-15N HSQC and 1H-13C HSQC). The 1H, 13C and 15N chemical shifts of the protein segment were assigned. The interaction between CtsR and clpC operon and the regulatory mechanism of arginine phosphorylation were analyzed.

Key words： transcription factor; nuclear magnetic resonance (NMR); chemical shifts; structure in solution

Cite this article

Xiao-wen CHEN , Bi-ling HUANG , Shao-hua HUANG , Yu-fen ZHAO . An NMR Study on the clpC Operon Binding Region of Transcription Factor CtsR from Bacillus subtilis[J]. Chinese Journal of Magnetic Resonance, 2021 , 38(2) : 155 -163 . DOI: 10.11938/cjmr20202854

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