Chinese Journal of Magnetic Resonance >
In Vivo Glutathione Molecular MRS Signal Selection Based on Nuclear Spin Singlet States
Received date: 2024-03-26
Online published: 2024-04-28
Glutathione (GSH), a tripeptide derived from glutamic acid, cysteine, and glycine, as a vital source of reducing power in the body, is crucial for maintaining redox balance and combating free radicals. Its role in tumor therapy is also significant. Monitoring GSH levels in vivo using magnetic resonance spectroscopy (MRS) is essential for understanding its biological functions. However, due to complex biological environments, overlapping problem of MRS signals is often serious, which makes selective detection and accurate quantification challenging. This paper introduces a method to selectively detect GSH in vivo by using nuclear spin singlet states. Compared to other methods in literatures, our method offers high efficiency and strong selectivity.
ZHU Xiangwei , YANG Xue , WEI Daxiu , YAO Yefeng . In Vivo Glutathione Molecular MRS Signal Selection Based on Nuclear Spin Singlet States[J]. Chinese Journal of Magnetic Resonance, 2024 , 41(4) : 373 -381 . DOI: 10.11938/cjmr20243105
| [1] | DRINGEN R, HIRRLINGER J. Glutathione pathways in the brain[J]. Biol Chem, 2003, 384(4): 505-516. |
| [2] | SONG J, KANG S M, LEE W T, et al. Glutathione protects brain endothelial cells from hydrogen peroxide-induced oxidative stress by increasing nrf2 expression[J]. Exp Neurobiol, 2014, 23(1): 93-103. |
| [3] | NIU B, LIAO K, ZHOU Y, et al. Application of glutathione depletion in cancer therapy: Enhanced ROS-based therapy, ferroptosis, and chemotherapy[J]. Biomaterials, 2021, 277: 121110. |
| [4] | KENNEDY L, SANDHU J K, HARPER M-E, et al. Role of glutathione in cancer: From mechanisms to therapies[J]. Biomolecules, 2020, 10(10): 1429. |
| [5] | VAIRETTI M, DI PASQUA L G, CAGNA M, et al. Changes in glutathione content in liver diseases: An update[J]. Antioxidants, 2021, 10(3): 364. |
| [6] | YUAN L, KAPLOWITZ N. Glutathione in liver diseases and hepatotoxicity[J]. Mol Aspects Med, 2009, 30(1): 29-41. |
| [7] | LIU H, HARRELL L E, SHENVI S, et al. Gender differences in glutathione metabolism in Alzheimer's disease[J]. J Neurosci Res, 2005, 79(6): 861-867. |
| [8] | DO K Q, TRABESINGER A H, KIRSTEN-KRüGER M, et al. Schizophrenia: glutathione deficit in cerebrospinal fluid and prefrontal cortex in vivo[J]. Eur J Neurosci, 2000, 12(10): 3721-3728. |
| [9] | KANTARCI K, JACK C R, XU Y C, et al. Regional metabolic patterns in mild cognitive impairment and Alzheimer’s disease[J]. Neurology, 2000, 55(2): 210-217. |
| [10] | VALENZUELA M J, SACHDEV P. Magnetic resonance spectroscopy in AD[J]. Neurology, 2001, 56(5): 592-598. |
| [11] | CHEN Q, KE J, CAI X, et al. GABA-induced motor improvement following acute cerebral infarction[J]. Am J Transl Res, 2020, 12(12): 7724-7736. |
| [12] | KIM H, THOMPSON R B, ALLEN P S. Enhancement of spectral editing efficacy of multiple quantum filters in in vivo proton magnetic resonance spectroscopy[J]. J Magn Reson, 2012, 223: 90-97. |
| [13] | MANDAL P K, SAHARAN S, TRIPATHI M, et al. Brain glutathione levels-A novel biomarker for mild cognitive impairment and Alzheimer’s disease[J]. Biol Psychiatry, 2015, 78(10): 702-710. |
| [14] | VIDYASAGAR R, FAZOLLAHI A, DESMOND P, et al. Single-session reproducibility of MR spectroscopy measures of glutathione in the mesial temporal lobe with MEGA-PRESS[J]. J Neuroimaging, 2024, 34(1):224-231. |
| [15] | CHOI I-Y, LEE P. Doubly selective multiple quantum chemical shift imaging and T1 relaxation time measurement of glutathione (GSH) in the human brain in vivo[J]. NMR Biomed, 2013, 26(1): 28-34. |
| [16] | CHOI C, ZHAO C, DIMITROV I, et al. Measurement of glutathione in human brain at 3T using an improved double quantum filter in vivo[J]. J Magn Reson, 2009, 198(2): 160-166. |
| [17] | DEVIENCE S J, WALSWORTH R L, ROSEN M S. Preparation of nuclear spin singlet states using spin-lock induced crossing[J]. Phys Rev Lett, 2013, 111(17): 173002. |
| [18] | PILEIO G. Singlet NMR methodology in two-spin-1/2 systems[J]. Prog Nucl Mag Res Sp, 2017, 98-99: 1-19. |
| [19] | PILEIO G, LEVITT M H. Theory of long-lived nuclear spin states in solution nuclear magnetic resonance. II. Singlet spin locking[J]. J Chem Phys, 2009, 130(21): 214501. |
| [20] | KHANEJA N, REISS T, KEHLET C, et al. Optimal control of coupled spin dynamics: design of NMR pulse sequences by gradient ascent algorithms[J]. J Magn Reson, 2005, 172(2): 296-305. |
| [21] | XIN J X, WEI D X, REN Y, et al. Distinguishing glutamate and glutamine in in vivo 1H MRS based on nuclear spin singlet order filtering[J]. Magn Reson Med, 2023, 89(5): 1728-1740. |
| [22] | YANG X, LIU Y, FU C X, et al. Selectively probing the magnetic resonance signals of γ-aminobutyric acid in human brains in vivo[J]. J Magn Reson Imaging, 2024, 59(3): 954-963. |
| [23] | WANG Z W, XIN J X, WEI D X, et al. Preparation efficiency of singlet states in multi-spin systems with different coupling configurations[J]. Chinese J Magn Reson, 2024, 41(1): 67-76. |
| 王子文, 辛家祥, 魏达秀, 等. 不同耦合构型多自旋体系单重态制备效率研究[J]. 波谱学杂志, 2024, 41(1): 67-76. | |
| [24] | HU K R, YANG X, HUANG Z M, et al. Preparing nuclear spin singlet state in a three-spin system and its application in 2D spectrum[J]. Chinese J Magn Reson, 2022, 39(1): 96-107. |
| 胡凯瑞, 杨雪, 黄志明, 等. 三自旋体系核自旋单重态的制备与单重态二维谱的实现[J]. 波谱学杂志, 2022, 39(1): 96-107. | |
| [25] | BOTTOMLEY P A. Spatial localization in NMR spectroscopy in vivo[J]. Ann N Y Acad Sci, 1987, 508(1): 333-348. |
| [26] | CARRAVETTA M, LEVITT M H. Long-lived nuclear spin states in high-field solution NMR[J]. J Am Chem Soc, 2004, 126(20): 6228-6229. |
| [27] | TAYLER M C, LEVITT M H. Singlet nuclear magnetic resonance of nearly-equivalent spins[J]. Phys Chem Chem Phys, 2011, 13(13): 5556-5560. |
| [28] | TRABESINGER A H, WEBER O M, DUC C O, et al. Detection of glutathione in the human brain in vivo by means of double quantum coherence filtering[J]. Magn Reson Med, 1999, 42(2): 283-289. |
| [29] | BOTTINO F, LUCIGNANI M, NAPOLITANO A, et al. In vivo brain GSH: MRS methods and clinical applications[J]. Antioxidants, 2021, 10(9): 1407. |
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