The detection of microsamples holds extensive application demands in fields such as environmental monitoring, biological sciences, and medicine. When conventional magnetic resonance probes are used to detect microsamples, the smaller filling factors lead to reduced signal-to-noise ratios (SNR) in magnetic resonance detection. Using microcoils comparable in size to the microsamples can significantly improve detection sensitivity and enhance SNR. Furthermore, due to the growing demand for broadband detection, there is a desire to reduce experimental steps during sample analysis and shorten detection times. However, currently available commercial probes are not designed for microsample detection and are relatively too complex to meet the requirements for efficient and convenient applications. To address these issues, this study designed a multilayer solenoid microcoil with excellent field uniformity, low inductance, and resistance. The coil was fabricated using printed circuit board (PCB) technology and tested on a 1.5 T MRI system magnet equipped with a self-developed spectrometer and broadband RF front end, where multiple nuclei (¹H, ²H, ⁷Li, ¹⁹F) were detected in 250 nL samples, demonstrating broadband capability and high sensitivity. The results highlight its potential for efficient and versatile microsample analysis.