Chinese Journal of Magnetic Resonance >

2026 , Vol. 43 >Issue 1: 37 - 45

DOI: https://doi.org/10.11938/cjmr20253164

Articles

Investigating the Factors Influencing Oxidative Modification of Human Cytochrome c Using Girard's Reagent T as an NMR Probe

  • ZHANG Guangqing ,
  • ZHAN Jianhua ,
  • XIAO Xiong ,
  • ZHU Qinjun ,
  • JIANG Bin ,
  • LIU Maili ,
  • ZHANG Xu
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  • 1. State Key Laboratory of Magnetic Resonance Spectroscopy and Imaging, Innovation Academy for Precision Measurement of Science and Technology, Chinese Academy of Sciences, Wuhan 430071, China
    2. University of Chinese Academy of Sciences, Beijing 100049, China
    3. Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology, Wuhan 430074, China
    4. Optics Valley Laboratory, Wuhan 430074, China

Received date: 2025-04-27

  Online published: 2025-05-12

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Abstract

Oxidative modification of cytochrome c (Cyt c) may influence the local conformation of protein, yet the mechanism by which structural alterations of Cyt c affect its degree of oxidative modification remains unclear. In this study, Girard’s reagent T (GRT) was employed as a nuclear magnetic resonance (NMR) probe to investigate the oxidative modification levels of human Cyt c under varying environmental conditions. Experimental results demonstrated that protecting lysine residues through reductive methylation effectively reduced protein oxidation. Partial unfolding of Cyt c was found to enhance its oxidative modification, while binding Cyt c with cardiolipin significantly increased the extent of oxidation. Additionally, other factors such as protein aggregation exhibited inhibitory effects on oxidative modification.

Key words： cytochrome c; oxidative modification; Girard’s reagent T; methylation; NMR

Cite this article

ZHANG Guangqing , ZHAN Jianhua , XIAO Xiong , ZHU Qinjun , JIANG Bin , LIU Maili , ZHANG Xu . Investigating the Factors Influencing Oxidative Modification of Human Cytochrome c Using Girard's Reagent T as an NMR Probe[J]. Chinese Journal of Magnetic Resonance, 2026 , 43(1) : 37 -45 . DOI: 10.11938/cjmr20253164

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