Chinese Journal of Magnetic Resonance >
19F NMR Chemical Shift-encoded cRGD-targeted Dual-modal Probe for Multicolor Imaging
Received date: 2025-11-05
Online published: 2026-08-11
Specific imaging of the tumor microenvironment is critical for precise cancer diagnosis, yet conventional single-modal imaging techniques struggle to combine high sensitivity with high spatial resolution. This study designed and synthesized a cRGD peptide-targeted, nitroreductase (NTR)-responsive hemicyanine-19F dual-modal probe, Cy-F-cRGD. Through systematic screening of different fluorine-containing groups, we found that an ortho-difluoro structure induced a remarkable 19F chemical shift change (ΔδF = 10.87). Concurrently, the hemicyanine unit exhibited an 80 nm red shift in absorption and a 90-fold fluorescence enhancement, achieving an optical “off-on” signal switch. This work offers a new design strategy for developing smart responsive multimodal probes.
LI Na , XIAO Long , LI Sha , LIU Yudun , JIA Yushu , ZHANG Lei , CHEN Shizhen . 19F NMR Chemical Shift-encoded cRGD-targeted Dual-modal Probe for Multicolor Imaging[J]. Chinese Journal of Magnetic Resonance, 2026 , 43(3) : 279 -290 . DOI: 10.11938/cjmr20253189
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