Chinese Journal of Magnetic Resonance >
A Biocompatible Gadolinium (III)-Poly (Aspartic Acid-Co-Phenylalanine) for Liver Magnetic Resonance Imaging Contrast Agent
Received date: 2015-01-30
Revised date: 2015-05-19
Online published: 2015-06-05
Supported by
The National Natural Science Foundation of China (20975097 and 21305134).
A new biocompatible gadolinium (III)-macromolecule (AP-EDA-DOTA-Gd) was developed as a magnetic resonance imaging (MRI) contrast agent. Poly (aspartic acid-cophenylalanine) was synthesized, modified via ethylenediamine, conjugated with 1,4,7,10-tetraazacyclododecane-1,4,7,10-tetraacetic acid (DOTA) and finally chelated gadolinium (III), yielding gadolinium (III)-based macromolecule (AP-EDA-DOTA-Gd). The hemolytic tests showed the hemocompatibility of this gadolinium (III)-based macromolecular conjugate. In vitro, AP-EDA-DOTA-Gd could be degraded, when it was incubated with cathepsin B in phosphate buffered solution (pH = 5.5). The T1-relaxivity (15.95 mmol–1·L·s–1) of AP-EDA-DOTA-Gd was 2.9 times of that (5.59 mmol–1·L·s–1) of the clinical MRI contrast agent (Gd-DOTA) at 1.5 T and 25 ℃. The liver enhancement of AP-EDA-DOTA-Gd was 63.5±6.1% during the maximum enhancement time (50-80 min), which was much better than that of Gd-DOTA (24.2±2.9%, 10-30 min). AP-EDA-DOTA-Gd was expected to be a potential liver MRI contrast agent.
Key words： MRI contrast agent; gadolinium (III)-macromolecule; liver; relaxivity
XIAO Yan1 , ZHAN You-yang1 , XUE Rong1 , LI Xiao-jing1* , PEI Feng-kui1 , FENG Jiang-hua2* , ZHAN Bo-han2 . A Biocompatible Gadolinium (III)-Poly (Aspartic Acid-Co-Phenylalanine) for Liver Magnetic Resonance Imaging Contrast Agent[J]. Chinese Journal of Magnetic Resonance, 2015 , 32(2) : 273 -282 . DOI: 10.11938/cjmr20150210
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