Chinese Journal of Magnetic Resonance >
Improvement of Fluorescence Quantum Yield for Terbium (III)-Bound Lanthanide-Binding Tag
Received date: 2015-04-13
Revised date: 2016-01-26
Online published: 2016-03-05
Long-lived luminescence lifetime and large Stokes shift of the lanthanide ion made it widely used in fluorescence detection. The lanthanide-binding tag (LBT) is a small, genetically encoded, versatile protein fusion partner that specifically binds a lanthanide ion with high affinity (KD ≈10-9 mol/L). These properties make LBT wildly used for studies on protein structures and functions. Lanthanide in LBT cannot be excited directly, and tryptophan is needed to serves as a sensitizer to absorb energy and transfer energy to the lanthanide. We introduced LBT onto the C-terminal of ubiquitin (Ub), and increased the number of indole rings in LBT using site-directed mutagenesis to increase the quantum yield of Tb (III)-bound Ub-LBT.
CHEN Shao-min , TANG Chun , GONG Zhou . Improvement of Fluorescence Quantum Yield for Terbium (III)-Bound Lanthanide-Binding Tag[J]. Chinese Journal of Magnetic Resonance, 2016 , 33(1) : 106 -116 . DOI: 10.11938/cjmr20160110
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