In this paper, the chemical structure of Nebmycin T was investigated by NMR. This method based on various kinds of NMR spectra, which included ¹H、¹³C、DEPT、COSY、R-COSY、LR-COSY、NOESY and 2D-J, is successful in determination of structure of Nebmycin T. The results showed that Nebmycin T is an aminoglucoside compound. Their configurations were assigned based on the 2D-J spectrum. We determined the positions of amino-groups in Nebmycin T with the solvent effect.

Calonyctin A which was extracted from leaves of Calonyction aculeatum (L.) House was discovered being not a single component. We extracted the sample from Calonyction aculeatum (L.) House then separated by HPLC and got two major components denominated as DC-2b and DC-2d.
In this paper, the structure of DC-2b was determined by ¹³C-NMR, DEPT, SELECTIVE INEPT, 2D ¹H-COSY, 2D ¹³C-¹H COSY and 2D-NOESY techniques.

A series of copolymers of β-Cyclodextrin and Epichlorohydrin were studied by means of ¹³C NMR, and spectral assignments were carried out. Their DEPT spectra checked the assignments correct. A group of α-substituted parameters of ¹³C NMR chemical shift at C(2)-O -, C(3)-O-and C(6)-O-positions of β-CD by 2,3-propanediol group was obtained. This paper investigated and described the compositions and the structural characterization of molecular chains in the copolymers.

The coordination behavior of Ascorbic Acid with metal ions Mn²⁺, Gd³⁺ in different solvents has been systematically studied. The NMR line broadening effect of paramagnetic ions on their adjacent carbon atoms has been found.

The ESR spectra of oxovanadium 1,10-phenanthroline and 3,4,7,8-tetramethyl-1,10-phcnanthroline complexes in glycol water(1:1) solution at various acidity (pH=1-14) have been observed at -100℃. It was found that within the range of 1.4 < pH < 6 phenanthroline can coordinate with oxovanadium to form three complexes and that within the range of 1.0 < pH < 7 3,4,7,8 -tetramethyl-1,10-phenanthroline can coordinate with oxovanadium to form four complexes. Their possible structures were suggested in this paper. When pH-4T is attributed to the complex[VO(H₂O) ₅]²⁺; when pH>11 A=90×10^-4T is attributed to the bidentate chelate of glycol[VO (OCH₂CH₂O)₂]^2- at a ratio of 1:2 of VO(Ⅱ):glycol. The bonding parameters and electronic energy levels were calculated using the spectral parameters measured. It was found that the values of bonding parameters decrease with the increasing of pH values of the solution. The data of electronic energy levels agree fairly well with the experimental, results.

The complete assignments of ¹³C NMR chemical shifts for 1β-acetoxy-2β (α-methylbutanoyloxy)-6α-hydroxy-8β,9α-dibenzoyloxy-β-dihydroaga -rofuran(A) and 1β-acetoxy-2β(α-methylbutanoyloxy)-6α-hydroxy-8β -(β-furancarbonyloxy) -9α-benzoyloxy -β-dihydroagarofuran(B), were carried out on the basis of ¹H-¹³C correlation(¹H-¹³C COSY) spectra. The weak coupling between 6-Ha and 7-He, and between 8 -He and 9-He, and the "W" long -range coupling between 7-He and 9-He for compounds A and B were also determined on the basis of ¹H-¹H long-range correlation (¹H -¹H COSYLR) spectra.

The resonances of haem ring methyl and other groups were assigned. The conformation of Alkaline Isomeride of Ferricytochrome C and some new points of view about this conformation have been put forward with the aid of 2D-NMR spectra.

TOCSY which provides relay informatoins overall protons in spin coupling network is a new development of RELAY. It is especially suitable for large molecules which have several independent spin systems.
In this paper, MLEV-17 sequence has been used for TOCSY both in homo-and heteronuciear coherence experiments and two polypeptides have been analysed.

¹¹³Cd NMR spectra have been obtained on several Cd-polyaminocarboxylic acid complexes Cd-N-(2-Hydroxycthyl) ethylene diaminetriacetate (Cd-HEDTA), Cd-1,2-Propanediaminetetraacetate (Cd-PDTA) and Cd-Diethylenetriaminepentaacetatc (Cd-DTPA). The spectra revealed that Cd-HEDTA and Cd-PDTA have the same solution structure as the Cd-EDTA. The two lines of Cd-PDTA spectrum come from its two isomers and are indicative of the existance of more strained structure having the axial CH₃ group. The large chemical shift of Cd-DTPA shows that it is seven coordinated because in that case, three nitrogen atoms rather than two are bound to Cd atom. ¹¹³Cd nuclear Ovcrhauscr effect, η, and spin lattice relaxation time were determined for Cd-DTPA and Cd-HEDTA and used in the analysis of the contribution to the relaxation mechanism from the dipolar interaction with protons.

The structure of several NASICON compounds prepared by hydrothermal crystallization were studied by solid -state high-resolution ³¹P and ²⁹Si MAS NMR. The state and distribution of phosphorus and silicon in NASICON'S framework are understood, and the change in chemical shifts of ³¹P and ²⁹Si caused by the substitution of lattice positions of atoms are explained. This is the first NMR investigation on the compounds of Na_1-xZr_2-xNb_x(PO₄)₃ system.

The ¹H、¹³C、¹⁴N NMR spectra of[Co(H₂O)₄(NCS)₂](18 -C-6) synthesized recently were studied. Its structure was proven. Furthermore, the structure was supported further by IR spectrum.

This article studies the solvent effect of benzene on dimethyl dimethylthiomethylenemalonate in carbon tetrachloride. It was found that chemical shifts of the methyl protons of the title compound and that of benzene move to upfield gradually, as the mole fraction of benzene in the mixed solvent increases, meanwhile the resonance absorption line of the solvent of benzene is a sharp single line. At the same time, it was found that the chemical shifts of the methyl protons of the title compound and that of benzene in the mixed solvent show linear relation ship with the mole fraction of benzene in the mixed solvent.

A new NMR technique, two-dimensional RELAY-HOHAHA NMR spectroscopy was developed. The results obtained showed that the efficiency of magnetization transfer among sugar residues of glycoside are much better for this method than that for 2D RELAY COSY and 2D HOHAHA experiments. The subspectra corresponding to individual sugar components were extracted even from overlapping proton resonance by taking the cross sections of 2D spectra para-llel to the F2 axis at anomeric proton resonance. Thus all the sugar proton resonances can be assigned to the individual sugar components. 2D RELAY-HOHAHA experiment in combination with NOE difference spectroscopy has been successfully used for determining the glycosidation position in an aglycome moiety and the sequence of sugar moiety in a natural glycoside without chemical degradation. The sensitivity for the present procedure based on proton NMR is much better than that for glycosidation shift based on ¹³C NMR.

The convolution difference technique has been applied to ESR spectra resolution enhancement and to deduction of the intrinsic width contribution from the overall linewidth in ESR imaging process. The convolution difference operation is examined and the change of ESR spectra before and after convolution difference is compared.

We give an experiment to measure the number density of hydrogen atoms by using the Pumping Magnetic Resonance Curve(PMRC). The number density from 9.2×10¹¹ to 1.6×10¹¹ (atoms/cm³) has been determined in the cell temperature region 25-61℃ and at a given r. f discharge power in our experiment. The number density of hydrogen atoms decreases as the cell temperature increases wtih a constant rf discharge power.