In this paper we present some results of high resolution NMR studies of hi-stones, protamines and their interaction with DNA.
Differences were found in the spectral characteristics of histones and protamines.
The spectra of protamines showed that the Arg-δCH₂ peak was rather strong while the Lys-εCH₂ peak was rather weak. The intensity of proton resonances of Val, lie and Leu-CH₃ in protamines was relatively low and weak.
When protamines interacted with DNA. the intensity of proton peaks of Arg-δCH₂ became weak,but the Arg-δCH₂ and Arg-βCH₂ proton peaks of protamines did not show any obvious change.

The high resolution NMR, UV,IR spectra of histone and it's fraction F₁ and F_2b were measured.
Our studies indicated that there were obvious differences amony tke spectra of histone, histone fraction F₁ and F_2b.

Linear poly(hydroxyethyl methacrylate) has been prepared by γ-radiation irradiation polymerization in 20%(v/v) alcohol solution.
Dyad, triad and pentad comonomer distributions for the polymer were observed in ¹³C NMR spectrum obtained at 20 MHz and 35℃ condition. Assignments were made by comparing observed intensity distribution for each carbon type with those calculated assuming Bernoullian statistical model. The results show that the polymerization process conform with that of Bernoullian.
The poly (hydroxyethyl metharylate) prepared by this method has a tactic triad content of 70.2% syndic., 27.2% hetero., and 2.6% iso..

Determination of the assignment and relative intensity of tritium in the fire specific labelling compounds (dihydro-Alprenolol. Abendazol. Etorphine. pyronaridine and cholor promazine)and General labelling selagine using ³H-NMR spectrometry is described. In addition, The triton-proton coupling codnstants(J_HT)-were measured by the ³H-NMR spectra of Cmethyl-³H] Thymifine and[4-³H] phenylalanine without decoupling. The "magnetically-equivalent" proton-proton coupling constants can be calculated from J_HT Values.

The ¹¹BNMR spin echoes in amorphous ferromagnetic alloy Fe₇₉B_15.5 Si_3.5C₂ were measured. Their shapes were found to be dependent on both the strength of RF field and the width of the pulses, and compared with the calculated results. The agreement between the measured and calculated results was reasonably well.

This paper reports on the ¹H-NMR and ¹³C-NMR spectral data of β、γ-un-saturate amides. On the basis of the NMR data the configurations of these co-mpoundsfare proposed The chemical shifts of the proton on the olefenic carbon of these compounds are calculated by empirical equation It is compared with the experimental results And the errors are discussed.

The basic procedure for the digital calculations of the po-vier pattern has been introduced. As a premise of computer simulations a mathematic formula' tion withmore synthesis and universality has been deduced. Following considerations are accepted in our program the weight treatment of the combination of absorptions of all transitions resulting in the total spectrum, the fluctuation of the quadrupole interaction gives rise to a broadening of single lines, this effect delivers different effects to the central transition and satellites. It is emphasized that a set of parameters describing the resonance absorptions are obtained by an optimum fit with the all-over pattern and the coincidence of those shifting behaviours of characteristic peaks as the external field changing.

Effects of ionic strength and ethanol content on the coordination structure of Cu(Ⅱ) complex of poly(4-vinylpyridine) partially quaternized by ethyl br-omide (EBQP₄VP) in aqueous solution were studied by riscometric, spectro-photometric, and electron spin resonance (ESR) techniques. Changes of ionic strength and ethanol content of Cu(Ⅱ)-polymer complexes lead to the conformation changes of the polymeric ligand chain. The structural changes in the coordination sphere of the Cu(Ⅱ)-polymer complexes were induced by the conformational changes of the polymeric ligand. Changes of the catalytic oxidation activity of hydroquinone may result from distortion of tetragonal symmefry of Cu(Ⅱ)-polymer complexes.

In the previous paper the equilibrium constants Keq for protonation of substituted pyridine bases in aqueous solution have been determined. In this paper the equilibrium constants Keq of various substituted quinolines hare been determined with the same method.
The experimental results showed that the equilibrium constants of quinol-ine bases were decreased or increased,when certain hydrogen on thfe quinoline ring was substituted with electron-attracting groups or electron-donating groups respectively. Thus, the electronic effect of substituents can be deduced from the Keq values.

An autoshim derice with unclosed loop and rerersed feedback has been made up by using of microprocessor,thus the resolution of a FX90Q spectrometer could be automatically adjusted and controlled.The resolution stability is within 0.10Hz. It is adapted for long term accumulation in a variety of PFT-NMR spectrometer.

The various anisotropic spin interactions which can be represented by the rank two tensors are decomposed into isotropic, symmetric, and antisymmetric constituents. This paper deals with the NMR properties of the antisymmetric constituent of chemical shift interaction. The antisymmetric constituent under MAS effects both the line position and the linewidth in second order. This contribution together with the effect from nonsecular terms of symmetric constituent determines the ultimate resolution of MAS spectra. The powder pattern caused by the above two effects under MAS is calculated.

We discribe theoretically in this paper,with the expansion of the Bessel functions, the spinning sidebands (SB) and the approaches to the SB suppression of inhomogeneuos interactions under MAS. The analysis reveals that severe spectral distortions will appear with the most popular SB suppression technique TOSS, when the ratio of the strength of inhomogeneuos interaction under investigation to the spinning speed is larger than 2. An experimental comparison of the existing appraoches of the SB suppression is also made in the paper.

In this paper,the splitting of energy level ofthe C_r³⁺ in K₃[C_r(CN)₆] under the external magnetic field H acting on the axes (a,b,c)of its Crystal wa firstly Calculated. Secondly,the corresponding Wave functions and finallys the relative transition probabilities of the extremely excited electrons were calculated.

The distribution of magnetic field of superconducting magnet and the design of shim coils for correcting field gradients are[described in detail. The precision and stability required for the coil power supplies, coil positions and dimensions are also evaluated.

The ¹³C-NMR spectra of some glycyrrizic triterpenoids, 24-hydroxyglabro -lide (l),24-hydroxyglabrolide diacetate (2),glabrolide acetate (3),and glycyr-rhetic acid (4) are discussed. A full asigment of the ¹³C-NMR signals of the-se compounds are completed.lt is shown that the configuration at C-18 can easily be recognized by inspecting the chemical shifts of two characteristic carbons, C-12 anb C-16. In particular, the signal of C-12 is diagnostically valuable. The shifts of these carbon originateb from the change of the D/E ring juction. The multiplicity of close spectra lines of these compounds are established by proton broad band decoupling, single frequency off-resonance dacoupling anb partially relaxed spectra (PRFT). When proper pulse interval time (r=0.36 sec) was selected, nonprotonated carbon, CH, CH₂,and CH₃, can be easily recognized by PRFT spectra.

A general procedure for the determination of the optimum instrumental parameters and the methods of sample preparation in qualitative and quantitative CMR analysis has been discussed. Four Couples of Solvent and relaxation regent are tried. The couple C₂D₂Cl₄/Fe(acac)₃ is the best. The minimum time of operation may be rebuced to about 1.0h and 4.0h for the qualitative and the qualitative analysis respectively for samples of medium molecular weight.

This paper introduces the applications of polarization transfer in analysi of ¹³C spectra, aad gives the program and method to accomplish DEPT on FX-series NMR spectrometers.