MRI示踪的纳米酶用于级联反应增强的免疫治疗

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隋美菊¹^,², 张磊¹^,², 王瑞芳¹^,², 骆盈盈¹, 李莎¹, 丘茂松¹, 徐秋怡¹^,², 陈代钦¹^,², 陈世桢¹^,²^,³^,^*(

), 周欣¹^,²^,³

MRI-traceable Nanoenzyme for Cascade Catalysis-enhanced Immunotherapy

SUI Meiju¹^,², ZHANG Lei¹^,², WANG Ruifang¹^,², LUO Yingying¹, LI Sha¹, QIU Maosong¹, XU Qiuyi¹^,², CHEN Daiqin¹^,², CHEN Shizhen¹^,²^,³^,^*(

), ZHOU Xin¹^,²^,³

图2. MH NPs的类酶催化性能. (a) MH NPs催化过程示意图；(b) GSH与不同浓度MH NPs（12.5、25、50、100和200 μg·mL^-1）反应后DTNB溶液的紫外-可见吸收光谱和照片；(c) H₂O₂与不同浓度MH NPs（0、10 、20 、30 、40和50 μg·mL^-1）反应O₂随时间的生成情况；(d) H₂O₂分别与MH NPs（90 μL, 25 μg·mL^-1）、Fe²⁺和H₂O反应的电子顺磁共振谱图；(e)不同反应体系[TMB、TMB + H₂O₂和TMB + H₂O₂ + MH NPs（10 μL, 0.1 mg·mL^-1）]的紫外-可见吸收光谱；(f)反应体系[TMB + H₂O₂ + MH NPs（10 μL, 0.1 mg·mL^-1）]在不同时间的紫外-可见吸收光谱

Fig. 2. The enzyme-like catalytic performance of MH NPs. (a) Schematic illustration of catalytic process of MH NPs; (b) UV-vis absorption spectra and photographs of DTNB after reaction of GSH with MH NPs solution at different concentrations (12.5, 25, 50, 100, and 200 μg·mL^-1) ; (c) The generation of O₂ over time after the reaction of H₂O₂ with MH NPs of different concentrations (0, 10, 20, 30, 40 and 50 μg·mL^-1) ; (d) ESR spectra of H₂O₂ reacting with MH NPs (90 μL, 25 μg·mL^-1), Fe²⁺ and H₂O, respectively; (e) UV-vis absorption spectra of different reaction systems [TMB, TMB + H₂O₂, TMB + H₂O₂ + MH NPs (10 μL, 0.1 mg·mL^-1)]; (f) UV-vis absorption spectra of reaction system [TMB + H₂O₂ + MH NPs (10 μL, 0.1 mg·mL^-1)] at different times