MRI示踪的纳米酶用于级联反应增强的免疫治疗

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隋美菊¹^,², 张磊¹^,², 王瑞芳¹^,², 骆盈盈¹, 李莎¹, 丘茂松¹, 徐秋怡¹^,², 陈代钦¹^,², 陈世桢¹^,²^,³^,^*(

), 周欣¹^,²^,³

MRI-traceable Nanoenzyme for Cascade Catalysis-enhanced Immunotherapy

SUI Meiju¹^,², ZHANG Lei¹^,², WANG Ruifang¹^,², LUO Yingying¹, LI Sha¹, QIU Maosong¹, XU Qiuyi¹^,², CHEN Daiqin¹^,², CHEN Shizhen¹^,²^,³^,^*(

), ZHOU Xin¹^,²^,³

图5. MH NPs介导光热作用增强的抗肿瘤催化治疗的体外评价. (a)不同处理后4T1细胞内ROS水平的共聚焦荧光图像（比例尺：100 μm）；(b)不同处理后4T1细胞内ROS水平的半定量分析；(c)不同处理后4T1细胞内LPO水平的共聚焦荧光图像（比例尺：100 μm）；(d)不同处理后4T1细胞内LPO水平的半定量分析；(e)不同处理后4T1细胞中STING通路相关蛋白表达的Western blot结果；(f)不同处理后4T1细胞中STING信号通路相关蛋白表达的半定量分析；(g)利用transwell系统探究不同处理对4T1细胞诱导的树突状细胞熟化情况示意图；(h)细胞上清液中IFN-β的表达水平；(i)细胞上清液中TNF-α的表达水平；(j)树突状细胞熟化水平的流式分析结果

Fig. 5. In vitro assessments of photothermal-enhanced anti-tumor catalytic therapy mediated by MH NPs. (a) Confocal fluorescence images of the cellular ROS level in 4T1 cells after different treatments (scale bar: 100 μm); (b) Semi-quantitative analysis of ROS level in 4T1 cells after different treatments; (c) Confocal fluorescence images of the cellular LPO level in 4T1 cells after different treatments (scale bar: 100 μm); (d) Semi-quantitative analysis of LPO level in 4T1 cells after different treatments; (e) Western blot assay of the STING signaling pathway-related protein expressed in 4T1 cells after different treatments; (f) Semi-quantitative analysis of the STING pathway-related protein expressed in 4T1 cells after different treatments; (g) The scheme of transwell system utilized to explore the maturation of dendritic cells induced by different treatments for 4T1 cells; (h) The expression level of IFN-β in the cell supernatant; (i) The expression level of TNF-α in the cell supernatant; (j) The level of mature dendritic cells measured by flow cytometry