HDX-NMR与HDX-MS在蛋白质结构动力学研究中的应用与进展

张媛媛, 汪鹏程, 李滔, 胡锐, 杨运煌, 刘买利

Development and Applications of HDX-NMR and HDX-MS in Protein Structure and Dynamics Research

ZHANG Yuanyuan, WANG Pengcheng, LI Tao, HU Rui, YANG Yunhuang, LIU Maili

图3 HDX-MS技术流程[21]，包括：样品制备、氢氘交换标记、淬灭、酶解、利用液相色谱（LC）分离多肽、质谱分析、氢氘交换分析，以及数据可视化呈现

Fig. 3 HDX-MS workflow[21], including sample preparation, HDX labeling, quenching, enzymatic digestion, peptide separation by liquid chromatography (LC), MS analysis, HDX analysis, and data presentation