HDX-NMR与HDX-MS在蛋白质结构动力学研究中的应用与进展

张媛媛, 汪鹏程, 李滔, 胡锐, 杨运煌, 刘买利

Development and Applications of HDX-NMR and HDX-MS in Protein Structure and Dynamics Research

ZHANG Yuanyuan, WANG Pengcheng, LI Tao, HU Rui, YANG Yunhuang, LIU Maili

图4 HDX-MS揭示INSM1与核酸结合的高度动态构象特征[43]. (a) HDX-MS数据镜像图，对比了INSM1 ZF1-ZF5游离状态（Apo）与结合M2 DNA状态（Holo）所检测到的各肽段的相对氘摄取量；(b)将Apo状态的各肽段在1 min时的相对氘摄取量映射至INSM1 ZF1-ZF5结构上；(c) HDX-MS数据差异图，展示了Apo与Holo状态INSM1 ZF1-ZF5之间相对氘摄取量的变化；(d)氢氘交换60 min时，INSM1 ZF1-ZF5中Apo与Holo状态间存在显著差异的片段

Fig. 4 HDX-MS reveals the highly dynamic conformational characteristics of INSM1 in binding to nucleic acids[43]. (a) A mirror plot of HDX-MS data comparing the relative deuterium uptake for each peptide of free INSM1 ZF1-ZF5 (Apo) and INSM1 ZF1-ZF5 bound by M2 DNA (Holo); (b) The relative deuterium uptake for each peptide of Apo INSM1 ZF1-ZF5 at 1 min was mapped on the structure of INSM1 ZF1-ZF5; (c) A difference plot of HDX-MS data showing the changes in relative deuterium uptake between Apo and Holo INSM1 ZF1-ZF5; (d) The segments of INSM1 ZF1-ZF5 with significant changes between Apo and Holo states at 60 min