以吉拉德试剂T作为NMR探针研究人源细胞色素c氧化修饰的影响因素

张广庆, 占建华, 肖雄, 朱勤俊, 蒋滨, 刘买利, 张许

Investigating the Factors Influencing Oxidative Modification of Human Cytochrome c Using Girard's Reagent T as an NMR Probe

ZHANG Guangqing, ZHAN Jianhua, XIAO Xiong, ZHU Qinjun, JIANG Bin, LIU Maili, ZHANG Xu

图6 (a) CL的1H NMR谱图；(b-d) Cyt c与6倍摩尔比的CL相互作用后在不同H2O2浓度下的下层清液1H NMR谱图；(e)图6(b)样品超滤后的上层和下层清液1H NMR谱图；(f) CL环境下Cyt c被H2O2氧化修饰的程度对比图

Fig 6 (a) 1H NMR spectrum of CL; (b-d) 1H NMR spectra of the subnatant following the interaction of Cyt c with a 6-fold molar excess of cardiolipin and subsequent treatment with varying concentrations of H2O2; (e) 1H NMR spectra of the retentate and filtrate fractions obtained after ultrafiltration of the sample shown in Figure 6(b); (f) Comparative analysis illustrating the degree of oxidative modification of Cyt c in the presence of CL under H2O2 stress.