蛋白冠原位调控增强肿瘤靶向19F MRI和联合治疗
In Situ Regulation of Protein Corona for Enhanced Tumor-targeted 19F MRI and Combined Therapy
蛋白冠原位调控增强肿瘤靶向19F MRI和联合治疗 |
| 李睿鹥, 李莎, 徐秋怡, 隋美菊, 陈世桢 |
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In Situ Regulation of Protein Corona for Enhanced Tumor-targeted 19F MRI and Combined Therapy |
| LI Ruiyi, LI Sha, XU Qiuyi, SUI Meiju, CHEN Shizhen |
| 图3 PP@BSA-TAFeIII NPs的体外性能评估. (a) PP@BSA-TAFeIII NPs溶液在不同pH下Fe3+释放的速度；(b) 808 nm激光（1 W·cm-2）照射下，不同浓度的PP@BSA-TAFeIII NPs溶液和H2O的升温曲线图；(c) 808 nm激光（1 W·cm-2）循环照射下，PP@BSA-TAFeIII NPs溶液的温度变化曲线；(d) PP@BSA-TAFeIII NPs（Probe）、PP@BSA-TA NPs、PP@BSA NPs分别与FBS孵育后的凝胶电泳图；(e) ELISA定量检测吸附在不同纳米颗粒上的转铁蛋白含量；(f)不同浓度PP@BSA-TAFeIII NPs溶液的19F MRI图像，及其信号强度与浓度的线性拟合曲线 |
| Fig. 3 In vitro characterization of PP@BSA-TAFeIII NPs. (a) pH-dependent release of Fe3+ by PP@BSA-TAFeIII NPs solution; (b) Temperature variation curves of PP@BSA-TAFeIII NPs solutions with different concentrations under the irradiation of 808 nm laser with 1.0 W·cm-2; (c) Temperature variation curves of PP@BSA-TAFeIII NPs solutions under the cyclic irradiation of 808 nm laser with 1.0 W·cm-2; (d) Gel electrophoresis of proteins adsorbed on PP@BSA-TAFeIII NPs (Probe), PP@BSA-TA NPs and PP@BSA NPs treated with FBS; (e) Quantitative detection of transferrin adsorbed on different nanoparticles by ELISA; (f) 19F MRI images of PP@BSA-TAFeIII NPs solutions at different concentrations, and the linear fitting curve of signal intensity versus concentration |
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