19F NMR化学位移编码的cRGD靶向双模态探针多色成像

李娜, 肖龙, 李莎, 刘昱墩, 贾宇舒, 张磊, 陈世桢

19F NMR Chemical Shift-encoded cRGD-targeted Dual-modal Probe for Multicolor Imaging

LI Na, XIAO Long, LI Sha, LIU Yudun, JIA Yushu, ZHANG Lei, CHEN Shizhen

图4 19F频率编码成像. (a)邻位（δ1、δ2）、间位（δ3、δ4）和对位（δ5、δ6）结构中间体纯样品的19F{1H} NMR谱图，中间体分子的浓度均为0.1 mmol/L，溶剂为2% DMSO + 98% H2O; (b)采样中心位δ1，采样带宽为5 400 Hz时δ1~δ6对应样品的溶液19F MRI; (c)采样带宽为1 000 Hz，激发频率设置为各样品中心时的19F MRI；(d) Cy-F-cRGD与NTR反应后随时间变化的19F MRI，激发中心分别设置为底物Cy-F-cRGD（δ1）和2,6-二氟苯胺（δ2）处. 所有溶液成像实验中探针浓度均为0.5 mmol/L，溶剂为5% DMSO + 95% H2O

Fig. 4 19F frequency-encoded imaging. (a) 19F{1H} NMR of pure intermediate molecule samples with ortho (δ1, δ2), meta (δ3, δ4), and para (δ5, δ6) substitution patterns. The concentration of intermediate molecules were 0.1 mmol/L, and the solvent were 2% DMSO + 98% H2O. (b) 19F MRI of samples δ1~δ6 acquired with the sampling center at δ1 and a bandwidth of 5 400 Hz. The sample concentration was 0.5 mmol/L. (c) 19F MRI acquired with a sampling bandwidth of 1 000 Hz and excitation frequencies set to the individual center frequencies of each sample. (d) Time-dependent 19F MRI of Cy-F-cRGD before and after reaction with NTR, with excitation centers set at δ1 (substrate Cy-F-cRGD) and δ2 (2,6-Difluoroaniline). In all solution imaging experiments, the concentration of probe was 0.5 mmol/L, with the solvent being 5% DMSO and 95% H2O