脉冲偶极耦合EPR技术在生物大分子结构变化与互作研究中的应用

谢雅欣, 杨茵, 苏循成

Applications of Pulsed Dipolar EPR Spectroscopy in Characterizing Interactions and Structural Changes of Biomacromolecules

XIE Yaxin, YANG Yin, SU Xuncheng

图2 偶极耦合脉冲EPR实验示意图. (a) DEER实验中泵浦脉冲与观测脉冲的位置关系示意图；(b) DQC和RIDME实验中的脉冲位置示意图；(c)实验中一对耦合电子自旋的空间构型示意，其中θ为自旋间距矢量$\overrightarrow{r}$与外加磁场$\overrightarrow{B0}$之间的夹角；(d)背景校正后的DEER时间轨迹、调制深度λ及最大偶极演化时间tmax的示意图

Fig. 2 Schematic illustration of pulsed dipolar EPR experiments. (a) Relative positions of pump and observer pulses in a DEER experiment; (b) Pulse position in DQC and RIDME experiments; (c) Geometry representation of a coupled electron spin pair, where θ denotes the angle between the inter-spin vector$\overrightarrow{r}$and the external magnetic field$\overrightarrow{B0}$; (d) Background-corrected time trace, modulation depth λ and maximum dipolar evolution time tmax obtained from a DEER experiment