Abstract: The distribution of hydrogen isotope-Deuterium in alcohol is determined by natural abundance Hyolrogen-2 Nuclear Magnetic Resonance, It is found that the content of Deuterium will be varied with different quality of water, And the deuterium conteut in methylene group varied more largely than in methylene group, But the difference of deuterium content of alcohol making with differeut material is shown predominantly in methyl group, Thus We obtained a significant method for monitoring quality of making compound and studing biochemical mechanism.