Abstract: ¹H spin system simulations of 2D-COSY spectrum of amino acid residues in insulin analogs were reported by using MARS program. From the comparison of the simulation spectra with the observed 2D-COSY spectra of Dcs-(B₂₆-B₃₀)-insulin and S-sulfonated insulin A and B chains, the cross peak patterns and fine structrues of simulated and obverved spectra were quite similar, indicating that the ¹H spin system identifications of residues from the observed 2D spectra arc correct, and on the other hand, the program MARS can be extensively used for the simulations of 2D-spectra of proteins.

Key words: 2D-NMR, 2D simulation spectra, Insulin