Abstract: The method of determination of the phase cycle in PFT-NMR experiments from the desired coherence transfer pathway was introduced. A computer program PHASE. BAS used to assist design phase cycling procedure based on this method was proposed. The program has been used to design a 128 step phase cycling procedure for the one and two dimensional INADEQUATE experiments. The one and two dimensional INADEQUATE spectra of n-Butanol were recorded by using this 128 step phase cycling procedure and a 32 step one collected in the Bruker MSL Pulse Sequence Library-INADSYMQ. PC respectively. The experimental results show that the former is superior to the latter in suppressing the single quantum signals and eliminating the imperfection of the spectrometer. It seems that the program could be an useful tool in the designing of pulse sequences, especially for the pulse sequences in which huge phase cycle steps are required.

Key words: Coherence transfer pathway, Phase cycle, INADEQUATE, Double-quantum correlation, N-Butanol