ESR STUDIES ON 2, 4, 6-TRINITROTOLUENE FREE RADICAL INTERMEDIATE INDUCED BY MICROSOMAL AND MITOCHONDRIAL NITROREDUCTASE

Chinese Journal of Magnetic Resonance ›› 1991, Vol. 8 ›› Issue (2): 167-172.

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ESR STUDIES ON 2, 4, 6-TRINITROTOLUENE FREE RADICAL INTERMEDIATE INDUCED BY MICROSOMAL AND MITOCHONDRIAL NITROREDUCTASE

Yang Zhenghong¹, Gao Mei¹, Wang Min², Zhuang Zhixiong²

1. Research Laboratories of Natural and Biomimctic Drugs, Mailbox 261;
2. Beijing Medical University, Beijing 100083

Received:1990-06-25 Revised:1990-09-06 Published:1991-06-05 Online:2018-01-20

Abstract

Abstract: Electron spin resonance spectra were observed during the enzymatic reduction of 2, 4, 6-Trinitrotoluene (TNT) respectively by rat hepatic and lens microsomes or mitochondria in anaerobic conditions at room temperature. With the evidence of ESR simulation of computer, the spectra indicated that TNT anion radical was present and its parameters are:g=2.0048±.0005, A (Npara)=1.215 mT, A (Northo)=0.800±.010 mT, A (H)=0.122±.020 mT, with 20mW of microwave power and 100 kHz of modulation frequency. The hyperfine splitting constants of TNT anion radical implied that the unpaired electron density in TNT was mainly distributed in para-nitro group.

Key words: TNT, ESR, TNT anion free radical

Yang Zhenghong, Gao Mei, Wang Min, Zhuang Zhixiong. ESR STUDIES ON 2, 4, 6-TRINITROTOLUENE FREE RADICAL INTERMEDIATE INDUCED BY MICROSOMAL AND MITOCHONDRIAL NITROREDUCTASE[J]. Chinese Journal of Magnetic Resonance, 1991, 8(2): 167-172.

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ESR STUDIES ON 2, 4, 6-TRINITROTOLUENE FREE RADICAL INTERMEDIATE INDUCED BY MICROSOMAL AND MITOCHONDRIAL NITROREDUCTASE

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