Abstract: Seven alkaloids have been isolated from Sophora flavescens Ait, which has its origin in Northeast China. Six of these alkaloids have been positively identified by MS, IR and 2D-NMR. These are (+)-matrine (1), (+)-oxymatrine (2), (+)-5α-hydroxymatrine (3), (-)-N-methylcytisine (4), (+)-9α-hydroxymatrine (5), and (+)-oxysophocarpine (7). The structure of the other alkaloid (6) is currently being determined. Two types of 2D-NMR H-H COSY (500MHz, 400MHz or 300MHz)and C-H COSY (C:75MHz, H:300MHz) techniques have been used.

Key words: 2D-NMR, Quinolizjdinc alkaloids, N-methylcytisine, Oxysophocarpine