DETERMINATION OF AMINO ACID SEQUENCES OF TWO NEW CYCLOPEPTIDES BY 2D NMR

Chinese Journal of Magnetic Resonance ›› 1993, Vol. 10 ›› Issue (1): 69-74.

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DETERMINATION OF AMINO ACID SEQUENCES OF TWO NEW CYCLOPEPTIDES BY 2D NMR

Tan Ninghua¹, Wang Dezu², Zhang Hongjie², Chen Changxiang², Zhou Jun², Zhao Shouxun¹

1. Department of Phyrochemistr China Pharmaceutical University, Nanjing, 210009;
2. Laboratory of Phytochcmistry, Kunming Institute of Botany, Academia Sinica, Kunming, 650204

Received:1992-06-09 Revised:1992-08-31 Published:1993-03-05 Online:2018-01-20

Abstract

Abstract: By combination of several NMR techniques (including ¹H-¹H、¹H-¹³C COSY、RELAY, and COLOC), the spin systems and sequences of amino acid residues of heterophyllin A and B, two new cyclopeptides which were isolated from Pseudostellaria heterophylla, have been successfully elucidated. The results indicated the sequences of amino acids with NH can be satisfactorily determined by COLOC when the ⁿJ_(XH) were 6Hz to 15Hz.

Key words: 2D NMR, COLOC, Determination of sequences of amino acids, Cyclopeptides, Heterophyllin A, Heterophyllin B

Tan Ninghua, Wang Dezu, Zhang Hongjie, Chen Changxiang, Zhou Jun, Zhao Shouxun. DETERMINATION OF AMINO ACID SEQUENCES OF TWO NEW CYCLOPEPTIDES BY 2D NMR[J]. Chinese Journal of Magnetic Resonance, 1993, 10(1): 69-74.

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DETERMINATION OF AMINO ACID SEQUENCES OF TWO NEW CYCLOPEPTIDES BY 2D NMR

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