IN VIVO 31P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results

Chinese Journal of Magnetic Resonance ›› 1994, Vol. 11 ›› Issue (3): 257-264.

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IN VIVO ³¹P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results

Yan Xianzhong¹, Li Hua², Feng Rui¹, Du Zehan¹, Ruan Jinxiu², Zhu Haoqin³

1. Academy of Military Medical Science, Beijing 100850;
2. China Pharmaeeutical University, Nanjing 210009

Received:1993-05-31 Published:1994-09-05 Online:2018-01-20

Abstract

Abstract: Metabolism of mice was studied by in vivo ³¹P NMR spectroscopy.spectra of brain,leg muscle and liver were obtained, using a two-turn surface coil.Peaks of PCr, ATP,PME PDE and Pi were observed and their Chemical shifts determined,The pH value of brain is 7.15, according to the chemical shift of Pi.The contaminated spectrum of liver was corrected by subtraction of a muscle spectrum from the observed spectrum.Metabolism of mice brain in hypoxia was studied.The pH value of brain decreased from 7.1 6 to 6.46 when mice died at the end of experiment.

Key words: in vivo NMR, Metabolism, Brain hypoxia

Yan Xianzhong, Li Hua, Feng Rui, Du Zehan, Ruan Jinxiu, Zhu Haoqin. IN VIVO ³¹P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results[J]. Chinese Journal of Magnetic Resonance, 1994, 11(3): 257-264.

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IN VIVO ³¹P NMR STUDIES OF METABOLISM IN ANIMALS——Methods and Preliminary Results

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