Abstract: In this paper we report on the result of study on aqueous solution of inclusion-complexesof host 2, 3, 6-tri-O-methylated β-Cyclodextrin with guest hydroquinone and cresols by ¹HNMR spectroscopy. The study shows that every four different substituted phenol can bebond by the host through inserting into the host hydrophobic cavity, and the correspondingbinding constant in aqueous solution can be calculated according to the relation betweenchemical shift changes of the host methoxyl protons and the concentration changing of thehost and different guests.

Key words: 2,3,6-tri-O-methylated β-Cyclodextrin, Hydroquinone, Cresols, ¹H NMR, Inclusion-complexion, Binding constant