Abstract: Three protopanaxodiol monodesmosides isolated from mild acid hydrolysis products of root saponins of Panax notoginseng were identified as ginsenoside-Rg5, 20(R)-ginsenoside-Rg3 and 20(S)-ginsenoside-Rg3. The complete assignments of ¹H and ¹³C NMR chemical shifts of these glycosides were obtained by means of 2D NMR techniques, such as ¹H-¹H COSY, TOCSY, ROESY, HMBC as well as HMQC. The differences of chemical shifts of 20(R)-and 20(S)-isomer were discussed.

Key words: Panax notoginseng, Protopanaxodiol saponins, NMR complete assignments