The results of ¹H NMR investigation of  β-Cyclodextrin inclusion complexes with three  cyanine dyes in D₂O and DMSO solution are reported in this paper, It was found that there is difference in chemical shift of some protons both of β-Cyclodextrin cavity and of cyanine dyes between after and before complexing,the position of which is dependent upon the inclusive degree. Fluorescence spectroscopy of cyanine dyes showed that the fluorescence intensity of these dyes is obviously quenched due to the forming of inclusions with β-Cyclodextrin, calculation results based on fluorescence spectroscopy indicated that β-Cyclodextrin formed 1∶1 inclusion with three cyanine dyes respectively. Information about hydrogen bond was induced from the study of temperature dependence of chemical shift of hydroxyl protons in β-Cyclodextrin cavity, OH-3 and OH-6. Their equilibrium constants of inclusion complexes were obtained from NMR chemical shift titration. The structure model of three inclusion complexes and dynamic process entering β-Cyclodextrin cavity has been described in this paper.

β-Cyclodextrin, Inclusion complex, Cyanine dye, Structural model