Chinese Journal of Magnetic Resonance

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A POWERFUL METHOD OF STUDYING METABOLISM IN BRAIN——High Resolution Nuclear Magnetic Resonance Spectroscopy

WANG Ke-qiang1, WANG Li-min1, XIA Wei2, YU Yi-hua2, GU Xing-hua1, JIA Jian-guo1, GE Jun-bo1   

  1. 1.The Medical Center of Fudan University (former Shanghai Medical University, Shanghai 200032);
    2.Shanghai Institute of Organis Chemistry, The Chinese Academy of Science, Shanghai 200032
  • Received:2001-01-08 Revised:2001-02-05 Published:2001-06-05 Online:2001-06-05

Abstract:

In this paper, a howerful method was introduced to study the metabolism in brain. Adult rats were frozen with liquid-N\-2 through a self-command funnel. A
fter perchloric acid (PCA) extracts were prepared and lyophilized, the residual substance were dissolved in D2O and investigated with 1H and 31P nuclear magnetic resonance spectroscopy (MRS). Result:High-resolution nuclear magnetic resonance spectroscopy of brain tissue was achieved. 31P MRS reveals ATP, ADP, phosphocreatine, inorganic orthophosphate and manifold aliphatic phosphates and sugar phosphates; 1H MRS shows many resonance peaks, including lactate, N-acetylaspartate, choline, creatine, GABA, myo-inositol, succinate and manifold amino acids. The peaks were integrated and the relative values might be used to express the metabolism or damnification of brain.

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