Abstract:

The rhizomes of \%Beesia calthaefolia\% yielded a triterpenic hexaglycoside  (1), the  structure of which was characterized by chemical and spectral means  (IR, ESI-MS, ¹H NMR, ¹³C NMR, ¹H-¹H COSY, ¹H -¹H TOCSY, HMQC, HMQC-TOCSY, HMBC) as oleanolic acid-3-O-β-D-gluco pyranosyl (1-3)-α-L-rhamnopyranosyl (1-2)-α-L-arabinopyranosyl-28-O-α-L-rhamnopyranosyl-(1-4)-β-D-glucopyranosyl (1-6)-β-D-glucopyranosyl ester. This paper discribes in detail the application of 2D NMR techniques in the structure el ucidation of this complex natural product.

Key words: Beesia calthaefolia, Spectroscopic analysis, 2D NMR, Oleanolic triterpenic glycoside